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slideserve.com
Gene Transduction via Lentiviral Vectors - SlideServe
Gene Transduction via Lentiviral Vectors. envelope (VSV-G). ‘vector’. gag/pol/rev. Transfect 293T cells with 3 pla smi ds that together create non-replicating lentivirus. The lentivirus can express a marker protein (here, GFP) as well as short hairpin sequences for RNAi.
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